Clinical features of thyroglossal duct carcinoma / 临床耳鼻咽喉头颈外科杂志

OJECTIVE@#To analyze the cinicopathological features, treatments, and prognosis of patients with papillary TDCa.@*METHOD@#A retrospective study was conducted of the medical records of our hospital for cases of TDCa. General clinical information including diagnostic criteria and treatments was obtained and analyzed. A literature review was also conducted.@*RESULT@#There were 160 cases of thyroglossal duct anomalies hospitalized in Peking Union Medical College Hospital in the past 20 years, and TDCa was diagnosed in 3 (1. 88%) cases. All 3 cases underwent local radical resections, and papillary TDCa was diagnosed based on the pathology examination. Selective neck dissection was chosen in one which was confirmed with cervical lymphatic metastasis. All patients were followed up with no recurrences or metastasis.@*CONCLUSION@#The incidence of TDCa is very low and the pathology examination is the only way to confirm the diagnosis. Surgery, especially Sistrunk's procedure, is the treatment of choice, with low complications and recurrence. In the presence of cervical lymphadenopathy, selective neck dissection should be considered.

Clinical features of primitive neuroectodermal tumor in head and neck / 中华耳鼻咽喉头颈外科杂志

<p><b>OBJECTIVE</b>To review the clinical characteristics, diagnosis and treatments of primitive neuroectodermal tumor (PNET) in head and neck.</p><p><b>METHODS</b>A retrospective review of the medical records for the cases of PNET in head and neck from 2004 to 2014. General clinical information including diagnosis and treatments was obtained and analyzed. A literature review was also conducted.</p><p><b>RESULTS</b>A total of 5 cases diagnosed with PNET were included. Of 5 patients, one patient with lesion in the temporal field was treated with radical resection, followed by radiotherapy, and a 24-month follow-up showed no recurrence. Three patients presented with maxillary, infratemporal fossa or cheek invasion respectively, after diagnosed with pathological examination, one patient received preoperation radiotherapy plus radical resection and postoperative chemotherapy, and the lesions had no obvious progress with follow-up of 106 months; one patient was applied with preoperative chemotherapy plus extensive resection and postoperation radiotherapy, showed recurrence 15 months later, and was lost to follow-up; and another patient underwent chemotherapy plus radiotherapy and extensive resection, presented with pulmonary metastasis 5 months later, and died of brain metastasis within 25 months. One foreign patient presented with tumor involved submaxillary, mouth and tongue, the tumor was reduced obviously after chemotherapy, but he was lost to follow-up after getting home.</p><p><b>CONCLUSIONS</b>PNET in head and neck is rare and the clinical profile of PNET is the presence of occupying and compression. The pathology examination is an only way to confirm the diagnosis of PNET. The combination of excision, radiotherapy and chemotherapy is the treatment choice.</p>

The combination characteristics of EHEC O157 ∶H7 intimin and it's mutant intiminN916Y with translocated intimin receptor / 中华微生物学和免疫学杂志

Objective To analyze the combination characteristics between Tir-IBD( intimin binding domain) and its ligand intimin or mutant intiminN916Y of EHEC O157 ∶H7.Methods The gene of TirIBD (tir-ibd) from EHEC O157 ∶H7 chromosome was cloned into pMD18-T vector.Thereafter,the amplified gene was cloned into prokaryotic expression plasmid pET-21 a (+).The recombinant pasmid pET-21 a( +)-tir-ibd was transformed into E.coli BL21 (DE3).After inducement,the protein Tir-IBD was successfully expressed and analyzed with SDS-PAGE and Western blot.It was purified by affinity chromatography and ionexchange chromatography and was coupled on the Ni-NTA chip of BIACore 3000.Then the ligand intimin and mutant intiminN916Y were flow through the chip and their combination characteristics were detected.Results In the present study,the gene of Tir-IBD(tir-ibd) was successfully cloned into pET-21a(+).The results of SDS-PAGE and Western blot assay showed that the protein was successfully expressed,which accounts for 15％ of total expression products,and its molecular weight was about 10×103.The purity was above 95％ after purification.After coupled on the Ni-NTA chip of BIACore 3000,their combination characteristics with ligand intimin and mutant intiminN916Y were successfully detected.The equilibrium binding constants Ka was obtained by fitting the data with the BIACore evaluation program ( Version 4.1 ).The result showed that the combination characteristics between Tir-IBD and intimin have some difference compared with that of mutant intiminN916Y and the difference is temperature dependent.Conclusion Tir-IBD of EHEC O157 ∶H7 was successfully constructed and purified.The method to analyze the combination characteristics between Tir-IBD and its ligand intimin or mutant was established.The combination characteristics between Tir-IBD and intimin or mutant intiminN916Y have some temperature dependent difference and the mutated amino acids residue is crucial for their receptor-ligand binding.

Clinical analysis of 28 patients with chronic rhino-sinusitis only characterized olfactory disorders / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To analyses the clinical characteristics of 28 chronic rhino-sinusitis patients only characterized olfactory disorders.@*METHOD@#Twenty-eight patients who have only olfactory disorder were diagnosed chronic rhino-sinusitis, among which 16 patients accepted intranasal budesonide for 15 days. All patients accepted CT scan, T&T test and olfactory event-related potentials test before and after treatment.@*RESULT@#(1) No difference was found between 21 patients ( 12 months) (P > 0.05), significant difference was found between maxillary sinus,ethmoid sinus and frontal sinus, sphenoid sinus in CT scan (P < 0.01). (2) Olfactory function improves after treatment (P < 0.01). Significant difference is found between 12 patients ( < or =12 months) and 4 patients (P < 0.01).@*CONCLUSION@#(1) Chronic rhino-sinusitis patients who have only olfactory disorder were found; (2) Intranasal budesonide treatment could improve olfactory functions of chronic rhino-sinusitis' patients.

Application of robotic surgery in otolaryngology-head and neck surgery / 基础医学与临床

Robotic Surgery is a novel minimally invasive surgery which is advanced from the endoscopic surgical techniques.This new technique has a promising future to be applied in clinics because of the distinct and compelling advantages compared with the traditional endoscopic surgery,including true three-dimensional endoscopic vision,increased freedom of movement of instruments and tremor filtration.We review the potential applications,advantages,limitations of the da Vinci Surgical System in otolaryngology.

Relationship of lipids disorder with sex and hypertension in diabetic patients / 中国实用内科杂志

Objective To investigate the prevalence of lipids disorder in patients with diabetes and the relationship of lipids disorder with sex and hypertension.Methods Totally 2 545 diabetic patients were analyzed for their different lipids disorder based on the clinical data.Results In the group of diabetic patients,1 663 cases were found with lipids disorder (65.3%),and females and males with lipids disorder were 69.6% and 61.9% respectively (P